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KMID : 0380020030180060490
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 6 p.490 ~ p.494
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Production and Chracteristics of Antifungal agents from Bacteria
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Kim Hyun-Su
Yook Young-Min
Yeo Soo-Hwan
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Abstract
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For the production of antifungal compound, strain B-1 was used as a strong producing strain among bacteria isolated from various soil samples. The optimum medium for the production of antifungal compound was PDB (potato starch 0.4%, dextrose 2％, pH5.1). The optimum conditions for the production of antifungal compound didn’t affect on the carbon and nitrogen sources. The produced compound showed broad antimicrobial activity to the tested strains such as five fungi and four bacteria. The optimum pH and temperature of the production antifungal compound were pH 5.0 and 28, respectively. Ether extrct (1/) of culture broth was confirmed inhibitory zone by the thin layer chromatography and plate assay. The antimicrobial compound was unstabled after heat (121) trsatment. Strain B-1 was mass cultured in a 5-liter tormentor, containing 3 liters of PDB medium at 28, pH 5.0, 120 (pm with aeration (1L/min).
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KEYWORD
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Antifungal compound, bacteria strain B-1, optimum culture condition, mass production
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